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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Grpel2 maintains cardiomyocyte survival in diabetic cardiomyopathy through DLST-mediated mitochondrial dysfunction: a proof-of-concept study

Fig. 4

Grpel2 overexpression attenuated HG-induced mitochondrial dysfunction in vitro. A, B Oxygen consumption rate (OCR) and associated quantitative analysis of mitochondrial respiratory function in NCMs (n = 4/group). C, D Flow cytometry analysis and quantification of mitochondrial membrane potential by JC-1 staining in NCMs (n = 4/group). E, F Flow cytometry analysis and quantification of mitochondrial ROS content by MitoSOX staining in NCMs (n = 4/group). G Quantification of total intracellular ROS intensity in NCMs (n = 4/group). H Quantitative analysis of glutathione peroxidase (GPx) content in NCMs (n = 4/group). I Quantitative analysis of the relative ATP content in NCMs (n = 4/group). J Quantification of relative caspase 3 activity in control or Grpel2-overexpressing NCMs under NG or HG conditions (n = 4/group). K, L Flow cytometry analysis and quantification of apoptotic cells by Annexin V-FITC and propidium iodide (PI) staining (n = 4/group). Data are presented as the mean ± SD. Data were analysed by one-way ANOVA, followed by Tukey’s post hoc test. *p < 0.05

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