Fig. 3
Grpel2 overexpression protected against cardiomyocyte mitochondrial dysfunction in the diabetic heart. A Representative transmission electron microscopy images of the left ventricle of mice (magnification: 28,000 × [28 K] and 98,000 × [98 K]). Scale bars: 1 μm (top) and 0.5 μm (bottom). B–E Quantitative analysis of the mean mitochondrial size (B), number of mitochondria per μm2 (C), number of mitochondrial cristae per mitochondrial area (D) and cristae area per mitochondrial area (E) (n = 6/group). F–H Western blotting and quantitative analysis of mitochondrial fission related proteins (Drp1and Fis1) and fusion-related proteins (Opa1, Mfn1 and Mfn2) in the left ventricle of mice (n = 4/group). I Representative images of MitoSOX staining (top) and DHE staining (bottom) of cardiac sections. Scale bars: 50 μm (up and down). J, K Quantitative analysis of relative mitoROS fluorescence (J) by MitoSOX staining and relative DHE fluorescence (K) by DHE staining (n = 6/group). L, M Quantitative analysis of glutathione peroxidase (GPx) content (L) and malondialdehyde (MDA) content (M) in the left ventricle of mice (n = 6/group). N Quantitative analysis of the relative ATP content in heart lysates (n = 6/group). Data are presented as the mean ± SD. Data were analysed by one-way ANOVA, followed by Tukey’s post hoc test. *p < 0.05