Fig. 4

Kaempferol up-regulated HSD17B1 expression and sensitivity in ER-negative EC cells. A For the AN3 CA cells, the mRNA expression of HSD17B1 was significantly decreased with 10 μg·mL⁻¹ treatment of kaempferol; for the HEC-1-A cells, the mRNA expression of HSD17B1 was significantly increased with 50 μg·mL⁻¹ treatment of kaempferol; the mRNA expression of HSD17B1 was barely expressed in KLE cells. B The protein expression level of HSD17B1 in ER-positive AN3 CA was unchanged, but the levels of HSD17B1 protein were significantly increased in ER-negative HEC-1-A with kaempferol treatment. And HSD17B1 was barely expressed in KLE cells. C–D Similar results were also found in the IHC of HSD17B1 in nude mouse tumor tissue after 3–7 weeks of treatment. E High expression levels of HSD17B1 had significantly shortened OS in EC patients. F Kaempferol may bind to the HSD17B1 hydrophobic LBD through several conserved hydrogen bond interactions with the amino acid residues Y155 and S142. G The AN3 CA cells were sensitive to kaempferol with a high level of HSD17B1. H Kaempferol can upregulate the expression of HSD17B1 in HEC-1-A. I Kaempferol-resistant KLE cells with low HSD17B1 expression