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Fig. 8 | Journal of Translational Medicine

Fig. 8

From: Atf4 regulates angiogenic differences between alveolar bone and long bone macrophages by regulating M1 polarization, based on single-cell RNA sequencing, RNA-seq and ATAC-seq analysis

Fig. 8

Atf4 regulating the angiogenic difference between ABM and LBM macrophages through M1 polarization. A WB analysis of iNOS and Atf4 in different groups. B PCR analysis of Vegfa in different groups. C WB analysis of Vegfa in different groups. D Quantification of the expression of Vegfa in ABM macrophages and LBM macrophages. E Tube formation effect of different CM on BMSCs (scale bar, 200 μm). F The plot of statistical analysis for numbers of meshes reflecting different effects of CM on BMSCs. G Tube formation effect of different CM on ECs (scale bar, 100 μm). H The plot of statistical analysis for numbers of meshes reflecting different effects of CM on ECs. *Corrected P-value < 0.05, **Corrected P-value < 0.01, ***Corrected P-value < 0.001. iNOS inducible nitric oxide synthase, Atf4 activating transcription factor 4, Vegfa vascular endothelial growth factor A, ABM alveolar bone marrow, LBM long bone marrow, WB western blot, PCR polymerase chain reaction, CM conditioned medium, BMSC bone mesenchymal stem cells, EC endothelial cell

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