Fig. 5

Identification of TF–target gene interactions in ABM and LBM macrophages using SCENIC and polarization status difference between the two. A The heatmap showed the enrichment of TFs of ABM and LBM macrophages. B In the tSNE dimension, the darker the AUC of the selected six TFs in each cell cluster, the higher the AUC value. C TF–target gene interaction network. D Expression of the six TFs in ABM macrophages and LBM macrophages. E Expression of the six TFs in ABM macrophages and LBM macrophages was analyzed by PCR assay. F Comparison of Atf4 expression in ABM macrophages and LBM macrophages by WB. G Quantification of the expression of Atf4 in ABM macrophages and LBM macrophages. H Comparison of iNOS, Arg-1, expression in ABM macrophages and LBM macrophages by WB. I Quantification of the expression of iNOS in ABM macrophages and LBM macrophages. J Quantification of the expression of Arg-1 in ABM macrophages and LBM macrophages. Data were expressed as mean ± SEM from 3 independent experiments. *Corrected P-value < 0.05. ***Corrected P-value < 0.001. TF transcription factor, ABM alveolar bone marrow, LBM long bone marrow, AUC area under the curve, Atf4 activating transcription factor 4, iNOS inducible nitric oxide synthase, Arg-1 arginase-1, PCR polymerase chain reaction, WB western blot