Fig. 5

Severe peripheral inflammation downregulated tight junction protein expression 3 days after ICH. A–C Western blot images and quantitative analysis of ZO-1 and CLDN5 expression. The data are expressed as the mean ± SD. ^# vs. the sham group, P < 0.05; ^a vs. the LPS group, P < 0.05; ^b vs. the LPS + sham group, P < 0.05; ^& vs. the ICH group, P < 0.05; ns: no significance. D, E Double immunofluorescence staining images and magnified representative pictures of ZO-1 (green) and CLDN5 (green) in endothelial cells (CD31, red). Scale bar = 50 μm. The arrows in the magnified images indicate regions in which tight junction protein levels are significantly decreased in vessels