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Fig. 10 | Journal of Translational Medicine

Fig. 10

From: CCL5/CCR5-mediated peripheral inflammation exacerbates blood‒brain barrier disruption after intracerebral hemorrhage in mice

Fig. 10

CRISPR-mediated JAK2 knockdown reversed cerebral damage induced by severe peripheral inflammation 3 days after ICH via inhibition of the JAK2/STAT3/MMP9 signaling pathway. A EB fluorescence images (scale bar = 50 μm) and trajectories in the OFT. According to preset parameters, the mouse trajectories are presented in three colors: black (low speed), green (middlzsxxdsse speed), and red (high speed). BE Western blot images and quantitative analysis of JAK2, p-STAT3, and MMP9 levels in the ipsilateral hemisphere in the sham, ICH + LPS + vehicle, ICH + LPS + rCCL5, ICH + LPS + rCCL5 + Ctr CRISPR, and ICH + LPS + rCCL5 + JAK2 CRISPR groups 3 days after ICH. G, H) Statistical analysis of average velocity and BMS scores. The data are expressed as the mean ± SD. # vs. the sham group, P < 0.05; d vs. the ICH + LPS + vehicle group, P < 0.05; e vs. the ICH + LPS + rCCL5 group, P < 0.05; f vs. the ICH + LPS + rCCL5 + Ctr CRISPR group, P < 0.05; ns: no significance. Ctr: control

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