Fig. 2

TMZ administration leads to significantly changed intestinal flora distribution AWorkflow of glioma orthotopic xenograft model construction and TMZ treatment. B The α-diversity indexes of Chao1, Shannon and Simpson of gut microbiota in mice before (Model) and after TMZ (TMZ) treatment. (C) The β-diversity indexes evaluated by weighted-wilcox of gut microbiota between Model and TMZ treated mice. D OPLS-DA score plot based on LC–MS ( +) (R²X = 0.262, R²Y = 0.972, Q² = 0.798) and LC–MS (-) (R²X = 0.642, R²Y = 0.867, Q² = 0.708) data. E Volcano plots of differential metabolites with fold change > 2 and p < 0.05 based on LC–MS ( +) and LC–MS (-) between Model and TMZ treated mice