Fig. 2

FCN1 is upregulated in PIBD and associated with immune response. A The expression level of FCN1 in the rectal biopsies from PIBD and non-IBD subjects of the GSE117993 RNA-seq dataset. The y axis represents log2-scaled normalized counts by applying the regularized log (rlog) algorithm in DESeq2. Upregulated FCN1 expression in the colon B and peripheral whole blood (C) from PIBD and non-IBD subjects of the GSE126124 microarray dataset. The y axis represents log2-scaled normalized gene expression by using the robust multichip average (RMA) algorithm. Unpaired t test was employed to calculate P values. D GO enrichment analysis of DEGs between PIBD and non-IBD subjects. The bar plot exhibits significantly enriched GO terms and the pie chart shows the frequency of FCN1 involved in top 100 enriched GO terms. E The bar plot of significantly enriched FCN1-related MeSH terms of DEGs in PIBD compared with non-IBD subjects. F The protein-protein interaction subnetwork of DEGs associated with FCN1 based on STRING database. G The heatmap visualizing the relative expression of FCN1-associated genes in the GSE117993 dataset. H ROC curves and their corresponding AUCs of FCN1, S100A8 and S100A9 (subunits of calprotectin) in the colon validation datasets