Fig. 3
From: Lactobacillus rhamnosus GG cell-free supernatant as a novel anti-cancer adjuvant
Cell cycle analyses reveal G2/M block upon LGG-SN treatment in cancer cells. HT-29, HCT-116, Caco-2, A375 treated with 0% v/v (CTRL), 90% v/v LGG-SN and 5 × 10–7 M Vincristine (VIN). A. Cell cycle bar plots: percentage of cells in G2/M (purple), S (green), G0/G1 (orange) and sub G1 (grey). B. q-RT-PCR analysis of Cyclin A (left), Cyclin B (middle), Cyclin D (right) expression in HT-29, HCT-116, Caco-2, and A375, GAPDH used as housekeeping, data are expressed as 2−ddCt compared to CTRL. N = 3. Values are presented as Mean ± SD. Statistical significance was analyzed using ordinary one-way ANOVA with Dunnett's multiple comparisons test (q-RT-PCR plots) and two-way ANOVA with Tukey's multiple comparisons test (cell cycle plots). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; no asterisk = not significant