Fig. 3

HIIT-associated methylation profiling of cardiac fibroblasts treated with heart failure patient (n = 3) serum pre- and post-HIIT. A. Volcano plot showing the differential gene methylation patterns in cells treated with heart failure patient serum pre- and post-HIIT. Genes with significant changes in methylation profiles after HIIT are shown in red dots. Others are shown in blue dots. B. The methylation severity of the significantly hypermethylated genes after HIIT in each chromosome is demonstrated. Lines between the chromosome and the heatmap indicate methylation hot spots in the chromosome. C. The acyl-CoA dehydrogenase very long chain (ACADVL) gene was found to be the most involved in the HIIT-associated DNA methylation ()