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Fig. 9 | Journal of Translational Medicine

Fig. 9

From: Targeting KK-LC-1 inhibits malignant biological behaviors of triple-negative breast cancer

Fig. 9

Z8 can inhibit the malignant biological behaviors of triple-negative breast cancer cells by MAL2/MUC1-C/PI3K/AKT/mTOR pathway. A, B Detection of MAL2, MUC1-C, PI3K, p-PI3K, AKT, p-AKT, mTOR, and p-mTOR expression in MDA-MB-231/WT and MDA-MB-231/Z8 cells using western blotting (**P < 0.01). MDA-MB-231/WT: DMSO treatment of MDA-MB-231 wild type cells. MDA-MB-231/Z8: Z8 treatment of MDA-MB-231 wild type cells. C, D Detection of MAL2, MUC1-C, PI3K, p-PI3K, AKT, p-AKT, mTOR, and p-mTOR expression in MDA-MB-468/WT and MDA-MB-468/Z8 cells using western blotting (**P < 0.01). MDA-MB-468/WT: DMSO treatment of MDA-MB-231 wild type cells. MDA-MB-468/Z8: Z8 treatment of MDA-MB-231 wild type cells. E Detection of KK-LC-1 mRNA expression in MDA-MB-231/WT and MDA-MB-231/Z8 cells using RT-qPCR (*P < 0.05). F Detection of KK-LC-1 mRNA expression in MDA-MB-468/WT and MDA-MB-468/Z8 cells using RT-qPCR (*P < 0.05). G Detection of KK-LC-1 expression in MDA-MB-231/WT and MDA-MB-231/Z8 cells using western blotting. H Detection of KK-LC-1 expression in MDA-MB-468/WT and MDA-MB-468/Z8 cells using western blotting. I Detection of KK-LC-1 intracellular localization in MDA-MB-231/WT and MDA-MB-231/Z8 cells using immunofluorescence assay. J Detection of KK-LC-1 intracellular localization in MDA-MB-468/WT and MDA-MB-468/Z8 cells using immunofluorescence assay. K Western blotting showing degradation of KK-LC-1 protein in MDA-MB-231/WT and MDA-MB-231/Z8 cells. L Western blotting showing degradation of KK-LC-1 protein in MDA-MB-468/WT and MDA-MB-468/Z8 cells

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