Fig. 3

KK-LC-1 regulates the malignant biological behaviors of triple-negative breast cancer. A Representative images of high and low KK-LC-1 expression. (Left: ×200 magnification. Right: ×400 magnification). B Analysis of KK-LC-1 protein expression effects on DFS in 481 breast cancer patients. C Analysis of KK-LC-1 protein expression effects on the OS of 481 patients with breast cancer. D Analysis of KK-LC-1 expression effects on DFS in 98 triple-negative breast cancer patients. E Analysis of KK-LC-1 protein expression effects on OS in 98 triple-negative breast cancer patients. F The expression levels of KK-LC-1 in MDA-MB-231, MDA-MB-468, MCF7, SKBR3, BT474, and MCF10A cell lines were detected by western blotting. G The expression of KK-LC-1 in the MDA-MB-231 cell line was silenced by lentivirus and verified using western blotting. H Proliferation of MDA-MB-231/NC and MDA-MB-231/KD cells detected using the CCK8 assay. I Transwell assay to detect the invasive ability of MDA-MB-231/NC and MDA-MB-231/KD cells. J Transwell assay to detect the migration ability of MDA-MB-231/NC and MDA-MB-231/KD cells. K Differential analysis of the scratch healing ability of MDA-MB-231/NC and MDA-MB-231/KD cells. L Detection of apoptosis in MDA-MB-231/NC and MDA-MB-231/KD cells using flow cytometry. M Cell cycle detection in MDA-MB-231/NC and MDA-MB-231/KD cells using flow cytometry. (*P < 0.05, **P < 0.01)