Table 1 Antibody sources and dilutions
Antibody | Source | Catalog | Dilution ratio |
|---|---|---|---|
Primary antibodies for Western blotting | |||
Rabbit anti-MLKL polyclonal antibody | Abcam | Ab172868 | 1:2000 |
Rabbit anti-phospho-MLKL phospho-S345 monoclonal antibody | Abcam | Ab196436 | 1:2000 |
Rabbit-anti-RIPK3 polyclonal antibody | Abcam | Ab62344 | 1:2000 |
Rabbit anti-phospho-RIPK3 phospho-S232 monoclonal antibody | Abcam | Ab195117 | 1:2000 |
Anti-IL-1β polyclonal antibody | R&D | AF-401-NA | 1:2000 |
Anti-DRP1 antibody | Abcam | Ab184247 | 1:2000 |
Anti-p-DRP1 (phospho S616) antibody | CST | 3455 | 1:2000 |
Rabbit-anti-TOM20 antibody | Proteintech | 11802-1-AP | 1:2000 |
Mouse-anti-OPA1 antibody | BD | 612606 | 1:2000 |
Rabbit-anti-MFN2 antibody | Proteintech | 12186-1-AP | 1:2000 |
Rabbit-anti-MTFP1 antibody | Proteintech | 14257-1-AP | 1:1000 |
Rabbit-anti-PGAM5 antibody | Proteintech | 28,445–1-AP | 1:3000 |
Anti-BAX antibody | CST | 2772 | 1:2000 |
Rabbit-anti-BCL2 antibody | Cohesion | CPA1095 | 1:1000 |
Rabbit-anti-caspase3 antibody | CST | 9662 | 1:2000 |
Rabbit-anti-caspase6 antibody | CST | 9762 | 1:2000 |
GSDMDC1 Antibody (H-11) | Santa Cruz | sc-393581 | 1:2000 |
Rabbit-anti-LC3A/B(D3U4C) antibody | CST | 12741 | 1:2000 |
Rabbit-anti-Pink1 antibody | Abcam | Ab23707 | 1:1000 |
Rabbit-anti-beclin1 antibody | CST | 3738 | 1:2000 |
Anti-mTOR monoclonal antibody | Proteintech | 66888-1-lg | 1:2000 |
Anti-phospho-mTOR (Ser2448) Antibody | Proteintech | 67778-1-lg | 1:2000 |
Rabbit-anti-EIF4E antibody | Proteintech | 11149-1-AP | 1:2000 |
Rabbit-anti-P-EIF4EBP1-S65 antibody | Proteintech | #12721 | 1:2000 |
Anti-α-tubulin monoclonal antibody | Servicebio | GB11200 | 1:10,000 |
Secondary antibodies for Western blotting | |||
HRP-conjugated goat anti-rabbit IgG | SAB | #L3012-2 | 1: 5000 |
Goat anti-Mouse IgG | SAB | L3032 | 1: 5000 |
Primary antibodies for immunofluorescence | |||
Rabbit anti-TOM20 polyclonal antibody | Proteintech | 11802-1-AP | 1: 200 |
CoraLite®488-conjugated MLKL antibody | Proteintech | CL488-66675 | 1: 300 |
Antibodies for Flow Cytometry | |||
PE-conjugated CD64 (FcγRI) | BioLegend | 139304 | 1:100 |
Fixable Viability Stain 450 | BioLegend | 562247 | 1:1000 |
APC-Cy7-conjugated CD45 (30-F11) | BioLegend | 557659 | 1:100 |
BV510-conjugated Siglec-F | BioLegend | 740158 | 1:100 |
APC-conjugated CD11b | BioLegend | 101212 | 1:100 |
Brilliant Violet 605-conjugated CD11c | BioLegend | 301636 | 1:100 |
PE/Cyanine7-conjugated F4-80 | BioLegend | 123114 | 1:100 |
CoraLite®488-conjugated MLKL antibody | Proteintech | CL488-66675 | 1: 200 |