Fig. 6

mTOR regulates TREM-1-induced necroptosis of macrophages. A the cell survival rate, n = 5. B–D Protein expression of p-RIPK3^ser232, RIPK3, p-MLKL^ser345, and MLKL, n = 3. E Immunofluorescence staining for MLKL (green) expression; scale bars = 5 μm. F The concentration of TNF‐α was assayed using ELISA, n = 3. G, H IL-1β p17 level in the supernatant, n = 3 biological replicates