Fig. 1

Priming NK cells with IL-12, IL-15, and IL-18 prompts trained memory-like phenotypic alterations among different NK subpopulations. A Schema of isolating NK cells from peripheral blood mononuclear cells and priming them with IL-12, IL-15, and IL-18 or low concentrations of IL-15 for 16 h, washing and then resting in a low concentration of IL-15 to allow for differentiation in vitro. B Representative flow plots showing the frequency of CD56 positive cells before and after positive separation. C Representative flow plots showing c-NK cells and CIML NK cells. Insert values are the percent positive of indicated markers for c-NK cells and CIML NK cells that fall within the indicated CD56 and CD16 gate. D The bar graphs show a comparison of c-NK and CIML NK cells from a representative individual on 3 NK cell phenotypic markers of different NK cell subsets. All values shown are mean ± s.e.m. of triplicate or duplicate measurements and have been repeated 3 times with similar results