Fig. 4

circDNAJC11 modulates BC cell apoptosis, cell cycle changes, and migratory and invasive capabilities. A–E BC cell apoptosis and cell cycle changes after circDNAJC11 knockdown were measured by flow cytometry. F–H After BC cells were transfected with corresponding siRNAs for 48 h, the expression levels of apoptosis-related proteins (Bax and Bcl-2) in BC cells were tested by western blot. F was the representative bands, and G and H were the statistical analysis. I and J Changes in migratory and invasive capabilities of MCF-7 and SK-BR-3 cells under different transfection conditions were analyzed by Transwell assay. I and J were the representative figures and the statistical analysis, respectively. Data were presented as mean ± SD and representative of three independent experiments in B, D, E, G, H, and J. For the above experiments, the Student's t-test was used for comparison between two groups, and the ANOVA test was used for three groups. * P < 0.05, ** P < 0.01, ***P < 0.001