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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: The spatiotemporal matching pattern of Ezrin/Periaxin involved in myoblast differentiation and fusion and Charcot-Marie-Tooth disease-associated muscle atrophy

Fig. 5

Ezrin is involved in myofiber specialization. A RNA-seq analysis of myofiber types in C2C12 myoblasts with overexpression or knockdown of Ezrin at 6 days. n = 3. B–E MyHC-I and MyHC-II fluorescence staining B–C and quantitative assay D, E for myoblast differentiation. Red fluorescence indicates MyHC-I (B: NOQ) or MyHC-II (C: MY32); DAPI indicates the nucleus. Three independent experiments were performed, n = 3, *P < 0.05 vs. Ad-Null; #P < 0.05 vs. Ad-Null. (F–I) In vivo assay for MyHC-1 expression in gastrocnemius (GA) and soleus (SL) muscles following the local injection of Ad-Ezrin. Red fluorescence indicates MyHC-I (F, G: NOQ); DAPI indicates the nucleus. n = 6, *P < 0.05 vs. Ad-Null

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