Fig. 2

Ezrin was specifically expressed in myoblasts and their skeletal muscle fibers. A Typical image of Ezrin expression in the gastrocnemius muscle. Red fluorescence indicates Ezrin, and DAPI indicates the nucleus. B Typical image of Ezrin expression in soleus muscles. Red fluorescence indicates Ezrin; green fluorescence indicates MyHC-I; DAPI indicates the nucleus. C Typical image of Ezrin expression in the gastrocnemius muscle. Red fluorescence indicates Ezrin; green fluorescence indicates MyHC-I; DAPI indicates the nucleus. D Quantitative assay for Ezrin expression in muscle. E The C2C12 myoblast differentiation model was established. Red fluorescence indicates MyHC; DAPI indicates the nucleus. F Ezrin expression was detected by Western blot at 0, 2, 4 and 6 days after myoblast differentiation. A quantitative assay for Ezrin expression was performed 2, 4 and 6 days after myoblast differentiation. Three independent experiments were performed, n = 3, *P < 0.05 vs. 0 days; ^#P < 0.05 vs. 2 days; ^&P < 0.05 vs. 4 days. G Typical image of Ezrin expression in the differentiaited myoblasts at 2, 4 and 6 days. Red fluorescence indicates Ezrin; DAPI indicates the nucleus. H Typical image of the colocalization of MyHC and Ezrin at 6 days after myoblast differentiation. Red fluorescence indicates MyHC; green fluorescence indicates Ezrin; DAPI indicates the nucleus