Fig. 3

VA effects on cell cycle regulatory proteins. Immunoblot of Cyclin D1 and p21 expression levels in MCF-7 cells (A) and Cyclin B1 and p21 in MDA-MB-231 cells (B) treated with VA for 24 and 48 h. GAPDH was used as a protein loading control. Histograms represent the mean ± SD of three experiments in which band intensities were evaluated in terms of arbitrary units of optical density (OD) and expressed as fold change relative to control *p<0.05 and **p<0.005 vs Control (C).