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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Nanoscale, antigen encounter-dependent, IL-12 delivery by CAR T cells plus PD-L1 blockade for cancer treatment

Fig. 2

A Schematic of sgRNA screening for the IL-12 subunits p40 and p35 performed in this study. B, C Inducible upregulation of IL-12—Non‐transduced (NT), cRB‐312, RB‐312_hh and RB-312_hm cells were stimulated at a 1:1 bead to cell ratio with beads coated with bovine serum albumin (BSA beads) and HER2 ectodomain (HER2 beads). qPCR and IL-12/p70 ELISA were performed to monitor IL-12 mRNA subunits and heterodimer upregulation after 3-day incubation with beads. D IFN‐γ secretion tested after 3-day incubation with beads. E, F NT, cRB‐312, RB‐312_hh, RB-312_hm and conventional HER2 CAR (convCAR) were co-cultured with FaDu cells at 3:1 effector to target (E:T) ratio and tested for kinetics of IL-12/p70 expression and IFN‐γ secretion. p values are ranked by the asterisks in all panels (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)

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