Fig. 1From: Modeling of Fabry disease nephropathy using patient derived human induced pluripotent stem cells and kidney organoid systemGeneration of hiPSCs using peripheral blood mononuclear cells derived from patients with Fabry disease nephropathy. A Light and electron microscopic findings of renal tissue biopsies from FD patients. On microscopic examination, there are some vague vacuolizations (black arrow) of podocytes (upper figure, H&E stain, ×400). Multi-lamellated myelin figures (red arrow), so-called zebra bodies as typical findings of FDN, are found in electron microscopy (lower figure). B Sequencing results of mutation in GLA gene from FD patients. C Schematic of iPSC reprogramming from patient by Sendai virus. D Immunofluorescence staining of three stem cell proteins (NANOG, SSEA-4, TRA-1-81). Scale bar = 50 μm, E Flow cytometry analysis of pluripotency markers (NANOG, SSEA-4 TRA-1-81) in cells. F Immunofluorescence staining of three germ layer markers: PAX4 for detecting ectoderm differentiation, SM22A for detecting mesoderm differentiation, and FOX2A for detecting endoderm differentiation. Scale bar = 50 μm. hiPSC, human pluripotent stem cell; FDN, Fabry disease nephropathyBack to article page