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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: FLI1 regulates radiotherapy resistance in nasopharyngeal carcinoma through TIE1-mediated PI3K/AKT signaling pathway

Fig. 3

Source data are provided as a Source Data file

FLI1 induces radioresistance by facilitating DNA double-strand breaks (DSBs) repair in NPC cells. A Immunofluorescence assays to examine the effect of FLI1 on γ-H2AX foci formation in the indicated CNE1 and 5-8F cells with or without IR. B Western blot assays of γ-H2AX, p-ATR, p-ATM, pCHK1 and p-CHK2 protein levels in the indicated CNE1 and 5-8F cells with or without IR. C Comet images and quantitative analysis of tail moments for DNA damage in the indicated CNE1 and 5-8F cells with or without IR treatment. D Western blot analysis of the time course of changes in γ-H2AX levels in NPC cells with 6 Gy IR exposure. Data in A and C are presented as mean ± SD (n = 3). **p < 0.01; ns not significant (Student’s t-test).

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