Fig. 2
Source data are provided as a Source Data file
FLI1 facilitates NPC cell survival after irradiation (IR) in vitro. A–B Western blot A and RT-qPCR B analysis of FLI1 protein and mRNA level in CNE1 cells with FLI1 overexpression and 5-8F cells with FLI1 knockdown (VEC, control lentiviral vector; FLI1, FLI1 overexpression lentiviral vector; NC, negative control shRNA; shFLI1, FLI1-specific shRNA). GAPDH was included as a loading control. C Cells were seeded at the density of 200, 400, 800 and 1000 cells for 0 Gy, 1 Gy, 2 Gy and 4 Gy IR dose. Colony formation assays and survival fraction curve analysis were employed to assess cell survival at 10–14 days after exposure to indicated IR dose. D–E Western blot D and Annexin V/PI double-staining E assays were performed to evaluate the effects of FLI1 on apoptosis 48 h after cells treated with or without IR. Data in B, C and E are presented as mean ± SD (n = 3). **p < 0.01, ***p < 0.001, ****p < 0.0001; ns not significant (Student’s t-test).