Fig. 8
From: P2RX7 promotes osteosarcoma progression and glucose metabolism by enhancing c-Myc stabilization
P2RX7 facilitated osteosarcoma growth and lung metastasis via c-Myc through metabolic reprogramming in vivo. a MNNG/HOS cells were transfected with empty vector (P2RX7 EV), P2RX7 plasmid (P2RX7 OE), EV plus MYC shRNA (P2RX7 EV + shMYC) and OE plus shMYC (P2RX7 OE + shMYC) respectively and then injected into mouse tibia. Stripped orthotopic osteosarcoma in tibia were shown and tumor weight were measured. b Bioluminescence images of tumor-bearing mice from a. The total luminous intensity of the region of interest (ROI) was shown. c Subcutaneous tumor model was established by injecting tumor cells transfected as in a. Tumor volume was monitored every 3 days and growth curve was then plotted. Tumor were weighed after isolation. d Lung tissue was resected in mice from a and metastatic nodules were counted. e Representative images of histological analysis, including Hematoxylin and Eosin (HE) staining and immunohistochemistry (IHC) staining with Ki67 antibody. Average optical density (AOD) of IHC was calculated. f Histological analysis of HE and IHC for lung tissue with metastasis. g Representative 18F-FDG PET/CT images of mice from a (n = 3). Arrows indicated tumor glucose uptake. h Lactate level of tumor tissues was detected in mice from a. Data are shown as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001 versus corresponding P2RX7 EV or OE group