Fig. 6

P2RX7 enhanced glucose metabolism via c-Myc. a, d Glucose consumption, glucose uptake, ATP production, pyruvate level, LDH activity and lactate level were examined in MNNG/HOS cells (a) and U-2 OS cells (d) transfected with empty vector (P2RX7 EV), P2RX7 plasmid (P2RX7 OE), EV plus MYC shRNA (P2RX7 EV + shMYC) and OE plus shMYC (P2RX7 OE + shMYC). b, e MNNG/HOS and U-2 OS cells were transfected as in a and extracellular acidification rate (ECAR) was then analyzed. c, f MNNG/HOS and U-2 OS cells were transfected as in a and oxygen consumption rate (OCR) was then determined. g Representative immunoblots of glycolytic protein expressions in osteosarcoma cells transfected as in a. h Representative immunoblots of oxidative phosphorylation-related protein expressions in osteosarcoma cells transfected as in a. Data are shown as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001 versus corresponding P2RX7 EV or OE group