Fig. 5
From: P2RX7 promotes osteosarcoma progression and glucose metabolism by enhancing c-Myc stabilization
P2RX7 promoted the stabilization of c-Myc via increasing nuclear retention and decreasing ubiquitination-mediated degradation. a, f The effect of P2RX7 on MYC gene expression assessed by qRT-PCR in osteosarcoma cells. b, g Immunoblot analysis was performed to estimate total c-Myc, phosphorylated c-MycS62 and c-MycT58 protein levels and the ratio of pS62/pT58 was then calculated. c, h Immunoblot of c-Myc expression in cytoplasm and nucleus. Lamin B and α-tubulin were used as internal controls for nuclear and cytoplasmic lysates, respectively. d, i Immunofluorescence showed the expression pattern of c-Myc in P2RX7 wild-type (WT) and knockout (KO) cells. Nuclei were counterstained with DAPI. e, j c-Myc expression was evaluated by immunoblot in P2RX7 WT and KO cells treated with DMSO or ubiquitination inhibitor MG132 (10 μM, 2 h). Data are shown as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001 versus WT counterpart