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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: P2RX7 promotes osteosarcoma progression and glucose metabolism by enhancing c-Myc stabilization

Fig. 4

P2RX7 facilitated osteosarcoma growth and lung metastasis through accelerating glucose metabolism in vivo. a P2RX7 wild-type (WT) and knockout (KO) MNNG/HOS cells were injected into mouse tibia to establish orthotopic osteosarcoma model. Tumor weights were recorded after mice sacrifice. b Bioluminescence images of tumor-bearing mice. The total luminous intensity of the region of interest (ROI) was presented. c Subcutaneous tumor was established and growth curve was plotted. Tumor were weighed after isolation. d Lung tissue was resected and metastatic nodules were counted. Lung weights were also measured. e Representative images of histological analysis, including Hematoxylin and Eosin (HE) staining and immunohistochemistry (IHC) with Ki67 antibody. Average optical density (AOD) of IHC was calculated and compared. f HE and IHC stainings in normal lung tissue. g Histological analysis for lung tissue with metastasis by HE and IHC. Data are shown as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001 versus corresponding P2RX7 WT group

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