Fig. 2

P2RX7 enhanced glycolysis and oxidative phosphorylation. a, d Glucose consumption, glucose uptake, ATP production, pyruvate level, LDH activity and lactate level were determined in P2RX7 wild-type (WT) and knockout (KO) MNNG/HOS cells (a), as well as in U-2 OS cells (d). b, e The analysis of extracellular acidification rate (ECAR) in MNNG/HOS cells (b) and U-2 OS cells (e), respectively. c, f The analysis of oxygen consumption rate (OCR) in MNNG/HOS cells (c) and U-2 OS cells (f), respectively. g Representative immunoblots of glycolytic proteins in P2RX7 WT and KO osteosarcoma cells. h Representative immunoblots of oxidative phosphorylation-related proteins in P2RX7 WT and KO osteosarcoma cells. Data are shown as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001 versus corresponding P2RX7 WT group