Fig. 1

P2RX7 regulated glucose metabolism in osteosarcoma. a Heatmap of glycolysis-related gene expression generated by RNA-seq in P2RX7 wild-type (WT) and knockout (KO) cells, respectively. qRT-PCR further confirmed the differential expression of glycolytic genes between P2RX7 WT and KO cells. b Heatmap of oxidative phosphorylation-related gene expression analyzed by RNA-seq in P2RX7 WT and KO cells, respectively. qRT-PCR further confirmed the differential expression of these genes between P2RX7 WT and KO cells. c Gene Set Enrichment Analysis (GSEA) analysis suggested significant difference of metabolic pathways between P2RX7 WT and KO cells. d Clustering heatmap of glucose metabolites by Quasi-targeted metabolomics between P2RX7 WT and KO MNNG/HOS cells. e Glucose uptake in tibia osteosarcoma through ¹⁸F-FDG PET/CT detection. Arrows indicated tumor glucose uptake. f Lactate level of tumor tissues from mice injected with P2RX7 WT and KO MNNG/HOS cells. Data are shown as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001 versus corresponding P2RX7 WT group