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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3

Fig. 4

The Progression of CRLM-IR regulated by hepatocytes SGK1 depends on the activation of STAT3. Using Gal-siSGK1or Gal-siCtrl and then a 90-min warm ischemia model was constructed in mice, and the liver was collected after 6 h reperfusion. A Immunohistochemical staining of p-STAT3 expression in ischemic livers or sham livers (n = 4 samples/group). Scale bars, 20 μm. B Quantification of p-STAT3 immunohistochemistry (n = 4 samples/group). C Immunofluorescence staining of p-STAT3 and HNF-4α expression in ischemic livers or sham livers (n = 4 samples/group), scale bars, 20 μm. D Quantification of p-STAT3 immunofluorescence (n = 4 samples/group). E Expression of p-STAT3 in primary hepatocytes was detected by flow cytometry (n = 3samples/group). F Flow cytometry detection of p-STAT3 expression in AML and LO2 after H/R (n = 3 samples/group). G The expression of SGK1, p-STAT3 and STAT3 in ischemic livers or sham livers was detected by western blot (n = 4 samples/group). H Quantification of p-STAT3/STAT3 relative intensity (n = 4 samples/group). I Immunohistochemistry staining for IL-6 expression in ischemic livers or control livers (n = 4 samples/group), scale bars, 50 μm. J Quantification of IL-6 immunohistochemistry (n = 4 samples/group). K Detection of mRNA levels of IL-6 in ischemic liver tissue or sham liver tissue by Quantitative RT-PCR (n = 6 samples/group). L The level of serum IL-6 in mice was detected by Elisa (n = 4 samples/group). M Detection of mRNA levels of IL-6 in hepatocytes isolated from ischemic liver lobes by Quantitative RT-PCR (n = 6 samples/group). N Western Blot detection of p-STAT3 and STAT3 in ischemic livers or sham liver treated with IL-6R antibody or control antibody (n = 3 samples/group). All data represent the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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