Fig. 2

Absence of hepatocyte SGK1 results in hepatic IR alleviation. 6–8 weeks wild type mice were first subjected to 70% warm ischemia, followed by reperfusion for 6 h. A Using Gal-siSGK1 or Gal-siCtrl, and the SGK1 expression was detected by western blot in primary hepatocytes or nonparenchymal cells isolated from ischemia livers or sham livers. SGK1 expression was also detected in ischemic liver tissue or sham liver tissue by western blot (n = 3 samples/group). B and C Liver function was evaluated by sALT and sAST levels (IU/L) (n = 3 samples/group). D Representative histological staining (H&E) of ischemic liver tissue (n = 6 samples/group). Scale bars, 100 μm. E Suzuki's histological score (n = 6 samples/group). F IR area calculated by Caseviewer (n = 5samples/group). G Apoptosis of liver cells was detected by tunel staining (n = 4 samples/group). Scale bars, 20 μm. H Quantification of apoptosis cells (n = 4 samples/group). I SGK1 expression of primary hepatocytes isolated from ischemic or sham livers was detected by flow cytometry (n = 3 samples/group). J ROS expression of primary hepatocytes isolated from ischemic or sham livers was detected by flow cytometry (n = 3samples/group). K Detection of ROS activation in LO2 and AML cell lines after H/R by flow cytometry (n = 3samples/group). All data represent the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001