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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3

Fig. 1

Hepatocyte SGK1 expression is up-regulated in response to hepatic ischemia reperfusion. 6–8 weeks mice were first subjected to 70% liver warm ischemia, followed by reperfusion for 3, 6, 12, 24, and 48 h. A and B Liver function was evaluated by sALT and sAST levels (U/L) (n = 3 samples/group). C Quantitative RT-PCR of SGK1 in ischemic livers or control group livers (n = 4 samples/group). D Immunofluorescence staining of SGK1 in ischemic livers or control livers, scale bars, 50 μm (n = 4 samples/group). E Mean fluorescence intensity of SGK1 (n = 4samples/group). F The expression of hepatocytes SGK1 was detected by western blotting (n = 4 samples/group). G Western blot analysis and relative intensity of SGK1 in hepatocytes (n = 4 samples/group). H Immunohistochemistry staining of SGK1 in ischemic livers or sham livers, scale bars, 50 μm. (n = 4 samples/group). I Quantification of hepatic SGK1 immunohistochemistry (n = 4 samples/group). J SGK1 expression in primary hepatocytes isolated from ischemic or control livers were detected by flow cytometry (n = 3 samples/group). K Detection of SGK1 expression in LO2 and AML cell lines after H/R by immunofluorescence (n = 3 samples/group). All data represent the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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