Fig. 3

HDAC inhibitor ITF2357 reduces the resistance of mut-KRAS NSCLC cells to Pem. A MTT assay was used to detect the viability of cells (A549, A549R) upon different treatments at different concentrations of Pem. B MTT assay was used to detect the viability of cells upon different treatments at different concentrations of ITF2357. C Transwell assay was used to detect the migration and invasion of cells upon different treatments (ITF2357 or Pem treatment alone, or their combination). D Flow cytometry was used to detect the apoptosis of cells upon different treatments (ITF2357 or Pem treatment alone, or their combination). *p < 0.05. **p < 0.01. ****p < 0.0001. Data among multiple groups were compared by one-way ANOVA, followed by Tukey’s post hoc tests. Data among multiple groups at different time points were compared by repeated measures ANOVA, followed by Bonferroni post hoc tests. Cellular experiments were repeated three times