Fig. 6
Effects of SRD5A1 silencing in T24 and J82 bladder cancer cell lines A, B Gene expression of SRD5A1 in shSRD5A1-transduced and control (Scr) cells was analyzed using reverse transcription-polymerase chain reaction (RT-PCR). The PCR products were run in an agarose gel (A) and the relative mRNA expression of SRD5A1 was normalized against GAPDH (B). N = 3. C The number of SRD5A1-silenced cells and control cells was counted after trypan blue exclusion for 3 days. N = 3. D Representative images from the colony forming unit assay and quantification of the number of colonies in shSRD5A1-transduced and control Scr cells. N = 3. E Cell closure analysis. The upper panels represent wound closure of control and SRD5A1-silenced cells, and the closure rates are indicated in the bottom panel. NT24 = 4. NJ82 = 3 F Migration analysis in T24 and J82 control and shSRD5A1 cells using transwell. The upper panels show representative images of the migration analysis and the relative migrated area is shown in the bottom panel. N = 3. All values are expressed as mean ± SD of at least three independent experiments, and statistical significance was analyzed using ordinary one-way ANOVA and two-way ANOVA. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)