Fig. 4

5α-reductase inhibition decreases the translational level of cancer progression-related proteins in bladder cancer cells treated with testosterone A, B Protein expression levels related to cancer survival and metastasis; p21, Bcl-2, matrix metalloproteinase-2 (MMP2), and MMP9 were analyzed using western blot. β-actin was used as an internal standard for band normalization. Western blot bands were measured and calculated using Image J. N = 3. C Graphs showing relative protein expression of Bcl-2 and P21 in T24 and J82 cells. D Graphs showing relative protein expression of MMP2 and MMP9 in T24 and J82 cells. E, F Protein expression levels related to NF-κB and GSK3β signaling pathways analyzed using western blot. β-actin was used as an internal standard for band normalization. Western blot bands were measured and calculated using Image J. N = 3. G Graphs showing relative protein expression of phospho-IκBα and phospho-p65 in T24 and J82 cells. Total IκBα and p65 were used as a standard for band normalization. H Graphs showing relative protein expression of phospho-GSK3β and β-catenin in T24 and J82 cells. Total GSK3β was used as a standard for band normalization. All values are shown as the mean ± SD of at least three independent experiments, and the statistical significance was analyzed using two-way ANOVA, indicated as *< 0.05, **< 0.01, ***< 0.001