Fig. 1

Androgen receptor expression and overexpression in bladder cancer cell lines A Representative image of an agarose gel after PCR amplification of the androgen receptor (AR) cDNA synthesized from RNA obtained from normal bladder and cancer cell lines. The graph shows the relative expression level of AR normalized against the housekeeping gene, GAPDH. Number of replicates (N) = 3. B Representative image of an agarose gel after PCR amplification of AR and SLC39A9 after lentiviral overexpression of AR in T24 cells. The graph shows the relative expression level normalized against GAPDH. N = 3. C Immunoblot probed against AR and β-actin from transduced T24 cells and graph showing the relative protein expression. D–G Cell viability assay in control (pLenti) and AR-overexpressed T24 cells visualized using EZ-cytox reagent. N = 3. H Representative images of the wound closure assay after AR overexpression in transduced-T24 cells. I Graphs showing the wound closure percentage measured using T scratch software. N = 3. The statistical analyses were compared using student t-test and ordinary two-way ANOVA. All values are estimated as mean ± SD of at least three independent experiments. The statistical significance; *< 0.05, **< 0.01, ***< 0.001, ns > 0.05