Fig. 9

Knockdown of NUDT1 causes mitochondrial dysfunction in GBM cells. A, B JC-1 dye was used to measure the change of MMP and the results were statistically analyzed by ImageJ software, scale bars: 50 μm. C The ATP levels in U251 and U87 cells. D, E After 24 h of transfection, the morphology of mitochondria was observed using representative confocal images of Mitotraker, scale bars: 1 μm. F TEM images were used to visualize the ultrastructure of mitochondria, scale bars: 1 μm. G After 24 h of transfection, WB was utilized to analyze the protein expression levels of MFN2, DRP1 and NUDT1. ** P < 0.01, *** P < 0.001