Fig. 2

Gain-of-function assays showed that LacRNA inhibits breast cancer metastasis both in vitro and in vivo. a Representative images of transwell migration assays for BT-549 cells overexpressing LINC00478_FL, LacRNA and LINC00478_3′RNA (scale bars, 50 μm). b Wound healing assay in BT549 cells overexpressing LINC00478_FL and LacRNA. c, d Representative images of bioluminescence imaging (BLI) of the lungs of NOD/SCID mice 7 weeks after orthotopic injection with vector pWPXL-LacRNA and control pWPXL-vector LM2 cells, n = 6. e, f Transwell migration and invasion assays of BT-549, MDA-MB-231 and T47D cells with LacRNA activation induced by CRISPR/dead-Cas9 technology. Values are expressed as the Mean ± SEM in a, b, e and f. Statistical significance was determined by one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, **** p < 0.0001. The stable cells established via the CRISPR/dead-Cas9 activation technology were designated as OE