Fig. 1

LacRNA is a small cytoplasm RNA derived from LINC00478 in breast cancer cells. a Schematic view of chromosomal location of LINC00478 and the 14 exons, 12 main alternative splices in UCSC browser. The alternative splices were divided into three groups, group A, group B and group C for further RT-qPCR analysis. RT-qPCR primers for each group were marked at the corresponding position of exons. b Relative RNA expression of 3 groups of LINC00478 isoforms assayed by RT-qPCR in MDA-MB-231, BT549 and T47D cells. Normalized to GAPDH. The data shown represent three independent experiments. c Relative RNA expression of 3 isoforms included in group C assayed by RT-qPCR in MDA-MB-231, BT549, T47D and LM2 cells. Normalized to GAPDH. The data shown represent three independent experiments. d RNA-seq data from UCSC browser of MCF-7 cell, showed the reads differently distributed in the last exon both in MCF-7 total cell and cytosol. e The 5′- and 3′- rapid amplification (5′/3′ RACE) and Sanger sequencing of cDNA ends of NR_136544, NR_136546 and NR_136545. Representative images of PCR products from the 5′/3′ RACE. Sequencing of RACE products are shown. f Half-life of LacRNA and LINC00478_3′RNA in MDA-MB-231 cells treated with 5 μg/ml actinomycin D for the indicated times. Myc served as the positive control. CCND1 served as the negative control. RNA was extracted and assayed by RT-qPCR. The data shown represent three independent experiments. g The relative expression of LacRNA, LINC00478_3′RNA and LINC00478_FL (LINC00478 full-length) in BT-549, MDA-MB-231, T47D and LM2 cells according to RT-qPCR. Mean ± SEM; n = 3 independent experiments