Fig. 4
From: Automating iPSC generation to enable autologous photoreceptor cell replacement therapy
Retinal differentiation of iPSCs generated on the Cell X platform. A Schematic of retinal differentiation protocol. Representative phase micrograph of day 0 iPSCs (B), day 7 EBs (C), day 16 attached optic vesicles (D), and day 55 lifted retinal organoids. Scale bar = 1 mm. Immunohistochemical staining of retinal organoids at day 120 (F– I) and day 160 (J–N). Antibodies targeted the photoreceptor cell markers OTX2 (green) and Recoverin (red), the rod photoreceptor cell markers NRL and NR2E3 (green) and cone photoreceptor cell marker ARR3 and ML-Opsin (red). DAPI (blue) was used as a nuclear counterstain