Fig. 6
From: Genetic dissection of the impact of lncRNA AI662270 during the development of atherosclerosis
AI662270 initiates cholesterol efflux and promotes foam cell formation. A, B Cholesterol efflux assay kit was used to measure cholesterol efflux to HDL or ApoA-I in peritoneal macrophages isolated from + AI662270-P (A) or + siAI662270 (B) (n = 5). C The total cholesterol content was assessed with a coupled enzyme assay (n = 5); **p < 0.01 vs. + NC-P, ##p < 0.01 vs. + siNC. D, E Oil red O staining was performed to verify the effect of AI662270 on foam cell formation in + AI662270-P (D) or + siAI662270 (E) (Scale bar = 100 µm, n = 5); **p < 0.01 vs. + NC-P or + siNC. “ + ” represents peritoneal macrophages cotreatment with ox-LDL (100 µg/mL). Data are expressed as mean ± SEM