Fig. 1

AI662270 is enriched in macrophages during atherogenesis. A Relative expression of AI662270 in various tissues of ApoE^−/− or WT mice with high fat diet (HFD) or chow diet (CD) (n = 6). B, C The effect of ox-LDL on AI662270 was confirmed by qRT-PCR (n = 6). D Cell counting kit-8 (CCK-8) was used to detect the effect of ox-LDL (100 µg/mL) on peritoneal macrophages (n = 6). E, H FISH was performed to monitor the expression and location of AI662270 in peritoneal macrophages (E) and aortic sinus (H), respectively (Scale bar = 20 μm, n = 6 for peritoneal macrophages; Scale bar = 200 μm, n = 3 for aortic sinus), “L” represents lumen. F Schematic representation of experimental mouse design. Briefly, 8-week-old male ApoE^−/− mice fed with HFD were intravenously injected with 100 μL recombinant lentivirus AI662270 (Lv-AI662270, 1 × 10⁸ UT/mL) or empty vector (Lv-null, 1 × 10⁸ UT/mL) for 16 weeks. G Transfection efficacy of Lv-AI662270 in aortas was measured by qRT-PCR (n = 6). **p < 0.01 vs. mice in CD or Lv-null treated mice in HFD in vivo, and Control (Ctr) in vitro. Data are expressed as mean ± SEM