Fig. 2

A: The distribution plot for the ratio of macrophages and Tregs shows individual samples colored according to the assigned K-means cluster. B: Parallel coordinate diagram of mean cluster values for macrophages and Tregs. Cluster 1 (red) is characterized by high Treg infiltration, cluster 2 (green) is characterized by high macrophage infiltration, cluster 3 (blue) has low Treg and low macrophage infiltration. C: Each line shows a representative case of the three clusters with HE-staining (magnification 7.2x) and multiplex immunohistochemistry for T-cell and TME markers: Nuclei were counterstained with DAPI (blue). T-cell panel: CD163 (cyan), CD4 (green), PD-1 (yellow), CD8 (orange), CD3 (red), FoxP3 (white); (B) TME panel: PanCK (cyan), αSMA (green), Vimentin (yellow), CD45 (orange), PD-L1 (red), Ki67 (white)