Fig. 4

Correlation of gut mycobiota with TNM stage and clinical physiological indicators in HCC patients. A Taxonomic cladogram from LEfSe showing differences in fecal taxa of HCC patients with stage III-IV and stage I-II. B LDA scores were computed for differentially abundant taxa in the gut fungi of HCC patients with stage III-IV and stage I-II. Length indicates the effect size associated with a taxon. P = 0.05 for the Kruskal-Wallis sum-rank test; LDA score > 4; C Spearman correlation analysis of fungal taxa and clinical physiological indicators in HCC patients. D Canonical correspondence analysis (CCA) of clinical indicators correlated with the fungal profile of patients with HCC. HCC hepatocellular carcinoma, LDA linear discriminant analysis, ALT alanine aminotransferase, AST aspartate aminotransferase, ALP alkaline phosphatase, GGT gamma-glutamyltransferase, TBIL total bilirubin, DBIL direct bilirubin, TBA total bile acid, RBC red blood cell, Hb hemoglobin, PA prealbumin, TP total protein, ALB albumin, GLB globulin, WBC white blood cell, NeuC neutrophil count, LYMC lymphocyte count, MonoC monocytes count, LMR monocyte-to-lymphocyte ratio, NLR neutrophil-lymphocyte ratio, PLT platelets, PT prothrombin, PT-act prothrombin time activity, PT-INR the international normalized ratio of prothrombin, APTT activated partial thromboplastin time, TT thrombin time, AT-III antithrombin III, *P < 0.05, **P < 0.01