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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Short term starvation potentiates the efficacy of chemotherapy in triple negative breast cancer via metabolic reprogramming

Fig. 4Fig. 4

Differential functional responses to starvation and chemotherapy in malignant and near normal breast cell lines. a Heatmap visualization of metabolism-related gene expression in MDA-MB-231, MCF-7, SKBR-3 and MCF-10A cells. Samples are arranged by treatment group. Each sample was measured in duplicate for each gene. Expression signature appears to be cell-specific and differentially affected by treatments. b Expression of ATP5A in breast epithelial cell lines upon STS, DXR or STS + DXR assessed by immunofluorescence analysis. Left panel: representative micrograph where ATP5A is shown in green, nuclei were counterstaining with DAPI (blue). Scale bar, 20 μm. Right panel: Quantification, where data are presented as mean corrected total cell fluorescence (CTCF) ± SD. *P ≤ 0.05. c ATP5A1 silencing mimicked the effect of STS + DXR treatment resulting in reduced cell viability in MDA-MB-231 and HS578, as demonstrated by lower Hoechst, higher PI and lower MTT signal in comparison to Scramble. FOXO1 silencing eliminate the selective sensitivity of combined STS + DXR treatment demonstrated by elevated cellular viability in MDA-MB-231 and HS578 cells as shown by higher Hoechst, lower PI and higher MTT signal in comparison to ‘Scramble RNAi STS + DXR’. d Assessment of intracellular ROS production in MCF-10A and MDA-MB-231 cells with or without ATP5A1 silencing. Data are presented as mean of the fold change ± SD. *P ≤ 0.05. e Assessment of intracellular ROS production in STS + DXR treated MCF-10A and MDA-MB-231 cells with or without FOXO1 silencing. Data are presented as mean of the fold change ± SD. *P ≤ 0.05

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