Fig. 3

The APA-induced 3′UTR lengthening promoted protein translation. a Scatterplots between ΔPDUI (DN-C) and expression changes in proteins (left) and mRNAs (right) levels for the mRNA-protein matched genes with significantly longer (ΔPDUI > 0.1, P-value ≤ 0.05) and shorter 3′UTRs (ΔPDUI < − 0.1, P-value ≤ 0.05). The genes were significantly upregulated (red) or downregulated (blue) (2-fold) in DN, respectively. b Scatterplots between ΔDPAU (DN-C) and expression changes in proteins (left) and mRNAs (right) levels for the mRNA-protein matched genes with significantly longer (ΔDPAU > 10, P-value ≤ 0.05) and shorter 3′UTRs (ΔDPAU < − 10, P-value ≤ 0.05). c The density plots of the statistically significant spearman correlation coefficient (P-value < 0.05) between PDUI (left) or DPAU (right) score and protein abundance for the mRNA-protein matched genes. The dashed lines represented the median value of positive (red) and negative (blue) correlation coefficient. d, e The experimental validation of the APA-mediated 3ʹUTR lengthening in DN increasing protein translation. Schematic diagrams of the pcDNA3.1(+)-CYB5R1-LUTR and pcDNA3.1(+)-CYB5R1-SUTR plasmid constructs were illustrated in d (left panel). The qRT-PCR (d, right panel) and western blot (e) of CYB5R1 in human podocytes under hyperglycemia were performed after transfecting cells with a plasmid expressing long or short 3′UTR isoform. The data represented the mean ± SEM of three independent experiments.