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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Loss of miR-26b-5p promotes gastric cancer progression via miR-26b-5p-PDE4B/CDK8-STAT3 feedback loop

Fig. 6

MiR-26b-5p regulates STAT3 signaling by targeting PDE4B and CDK8. A–B Total STAT3 expression and phosphorylation were detected by Western blot in MGC803 and HGC27 cells transfected with miR-26b-5p mimics, inhibitor, or controls. C–D MGC803 cells transfected with miR-26b-5p mimics or control mimics were treated with IL-6 (50 ng/mL) for 30 min before immunofluorescent staining for phosphorylated STAT3 (green). The nuclei were stained with DAPI (blue). E IHC of phosphorylated STAT3 in xenograft tumors of nude mice infected with miR-26 agomir, miR-26b-5p antagomir, or negative control cells (LV miR-26b-5p, anti-miR-26b-5p, Ctrl, and anti-NC). F MGC803 cells post-transfection with miR-26b-5p mimics or control mimics for 48 h were treated with different concentrations of IL-6 for 30 min. Total STAT3 expression and phosphorylation were examined by immunoblot. G MGC803 cells were transfected with miR-26b-5p mimics and CDK8 overexpression plasmids or empty vectors. I HGC27 cells were transfected with miR-26b-5p inhibitor and CDK8 siRNAs or siNC. CDK8, total STAT3, and STAT3 phosphorylation expression were detected by Western blot. H MGC803 cells were transfected with miR-26b-5p mimics and PDE4B overexpression plasmids or empty vectors. J HGC27 cells were transfected with miR-26b-5p inhibitor and PDE4B siRNAs or siNC. PDE4B, total STAT3, and STAT3 phosphorylation expression were examined using Western blot. Quantitative data are shown as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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