Fig. 4
From: Loss of miR-26b-5p promotes gastric cancer progression via miR-26b-5p-PDE4B/CDK8-STAT3 feedback loop
miR-26b-5p modulates cell growth by targeting PDE4B and CDK8. A–B Analyzing the expression of PDE4B and CDK8 in GC cell lines and GES1 cells by qRT-PCR. C Knockdown efficiency of PDE4B and CDK8 siRNAs. CCK8 D, colony formation, and EdU assays E–G were conducted to detect the effects of PDE4B and CDK8 silencing on HGC27 cell proliferation. HGC27 cells were transfected with miR-26b-5p mimics or miR-26b-5p mimics in combination with PDE4B or CDK8 overexpression vectors. Colony formation, EdU H–J, and CCK8 K assays were performed with the above cells to investigate whether PDE4B and CDK8 interfere with the function of miR-26b-5p. Quantitative data are shown as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001