Fig. 2

Suppression of miR-26b-5p promotes GC proliferation in vitro and in vivo. A qRT-PCR quantified the transfection efficiency of miR-26b-5p inhibitor in MGC803 and HGC27 cells. CCK8 B–C, colony formation D–E, and EdU F–H assays were conducted to assess the proliferation of MGC803 and HGC27 cells transfected with miR-26b-5p inhibitor or control. I Flow cytometry analysis of MGC803 and HGC27 cells transfected with miR-26b-5p inhibitor or control. J Pictures of xenograft tumors from nude mice 24 days after injection of miR-26b-5p agomir, miR-26b-5p antagomir, and their negative controls (LV miR-26b-5p, anti-miR-26b-5p, Ctrl, and anti-NC). K–L Tumor weight of xenografts was measured at the endpoint. M–N Tumor volume of xenografts was measured every 2 or 3 days and shown. O IHC of Ki67 in xenograft tumors. Quantitative data are shown as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001